Scientific Research References

J.Y. Oh et al, 2014

Peppermint Oil Promotes Hair Growth without Toxic Signs

This study investigated the effect of peppermint oil on hair growth in C57BL/6 mice. The animals were randomized into 4 groups based on different topical applications: saline (SA), jojoba oil (JO), 3% minoxidil (MXD), and 3% peppermint oil (PEO). The hair growth effects of the 4-week topical applications were evaluated in terms of hair growth, histological analysis, enzymatic activ- ity of alkaline phosphatase (ALP), and gene expression of insulin-like growth factor-1 (IGF-1), known bio-markers for the enhanced hair growth. Of the 4 experimental groups, PEO group showed the most prominent hair growth effects; a significant increase in dermal thickness, follicle number, and follicle depth. ALP activity and IGF-1 expression also significantly increased in PEO group. Body weight gain and food efficiency were not significantly different between groups. These results suggest that PEO induces a rapid anagen stage and could be used for a practical agent for hair growth without change of body weight gain and food efficiency.

Joshi and Dyawarkonda, 2017

Formulation and evaluation of polyherbal hair oil  

This study aimed at reviewing the importance of polyherbal hair oil for the treatment of common hair problems such as baldness, alopecia, hair fall, gray hair, dryness, and most common dandruff. Materials and Methods: The various herbal ingredients are used in the formulation are: Amla, Bhringraj, Yashtimadhu, Triphala, Henna, Neem, Aloe vera, hibiscus flowers, coconut oil, cow milk, grated coconut, and water. All ingredients provide essential nutrients such as vitamin, antioxidant, protein, terpenoids, and many essential oils to maintain normal function of sebaceous glands. Procedure for oil preparation is divided into two parts: (1) preparation of decoction of all the herbs and (2) oil preparation. Results and Discussion: Excellent results of hair growth were seen in formulation prepared by the abovementioned procedure. Formulated herbal oil was evaluated for various parameters such as specific gravity, viscosity, acid value, saponification value, pH, and irritation tests. Conclusion: In general, herbal formulation provides good blend of vitamins, antioxidants, terpenoids, and essential oils. All the values in the evaluation of finished product showed that they are within the acceptable limits. Hence, it is concluded that the oil is beneficial in maintaining good growth of hairs, turning gray hairs to black, providing protection from dandruff, and results in lustrous hairs.

Sundaram and Suresh, 2019

Prevention of hair fall and whitening of hair by valuable medicinal plants   

An ethnobotanical study was investigated in Madurai District which is mainly focused on prevention of hair fall and whitening hair by medicinal plants. The ancestral traditional knowledge of people who have been using the native plants for the preparation of drugs and methods of their administration along with doses were recorded, Collected through the questionnaire as well as informal personal interviews during field trips. The practical knowledge of people in herbal medicines reveals that they are capable of curing Hair related problems. The fruit of Medicinal plants such as Phyllanthus emblica (amla or indian gooseberry) among others used in hair loss prevent premature greying of Hair.

Ezekewe; King and Hollinger, 2020

The Use of Natural Ingredients in the Treatment of Alopecias with an Emphasis on Central Centrifugal Cicatricial Alopecia: A Systematic Review    

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W. N. Shebaby et al, 2015

Antioxidant and hepatoprotective activities of the oil fractions from wild carrot (Daucus carota ssp. carota)


Context: Wild carrot, Daucus carota L. ssp. carota (Apiacae), is widely distributed throughout the world and has various uses in traditional medicine in Lebanon.
Objective: The present study aimed to fractionate and analyze the chemical composition of the Daucus carota oil extract (DCOE) fractions and to evaluate their antioxidant and hepatopro- tective properties in vitro and in vivo.

Results: GCMS analysis of F2 revealed the presence of 2-himachalen-6-ol (61.4%) which is reported for the first time in Daucus carota species. F3 and F4 were rich in phenolics and flavonoids and demonstrated significant DPPH activity (IC50 1⁄4 0.29 and 0.38 mg/ml, respectively) and high FRAP values (225.11 and 437.59 mmol FeSO4/g, respectively). The sesquiterpene-rich fraction F1 had the highest FIC ability (IC50 1⁄4 0.28 mg/ml). Pretreatment with F1 and F4 reversed the CCl4-induced decrease in SOD, CAT, and GST levels and reduced significantly hepatic damage.

Discussion and conclusion: The current results suggested that wild carrot oil fractions exhibited a unique chemical composition and possessed significant antioxidant activities as well as hepatoprotective effects against CCl4-induced hepatotoxicity.

del Campo; Zhang and Wakeford, 2017

Effect of Miracle Fruit (Synsepalum dulcificum) Seed Oil (MFSO®) on the Measurable Improvement of Hair Breakage in Women with Damaged Hair: A Randomized, Double-blind, Placebo- controlled, Eight-month Trial


Objective: To assess the safety and efficacy of a hair oil containing MFSO and its effects on hair breakage rates. Methods: Healthy, long-haired women (age range: 19–63 years, mean age: 36.7 years, standard deviation: 10.77 years) with excessive hair breakage were randomized in this double-blind, placebo-controlled study to receive MFSO (n=24), vehicle (n=17), or argan oil (n=16). Measurements of hair length, hair diameter, and Hair Mass Index were performed at baseline, Month 4, and Month 8. Hair Breakage Index and the Healthy Hair Index values were calculated from the trichometer measurements, and subject self-assessment questionnaires were conducted. The primary efficacy endpoints were the percent change in Healthy Hair Index 75 and Healthy Hair Index 50 measurements from baseline to the eighth month. Results: The Healthy Hair Index calculations, expressed as percent change from baseline to Month 4 and from baseline to Month 8, revealed that the MFSO® treatment group improved by 103.6 percent and 215.7 percent for the Healthy Hair Index 75 and 133.7 and 188.3 percent for the Healthy Hair Index 50 values, respectively. When compared with the vehicle and the argan oil brand groups, the Healthy Hair Index levels were significantly higher (p < 0.001) for the MFSO® treatment group, indicating a much greater ability to increase the levels of unbroken hairs by reducing hair breakage. With respect to the mean percent improvements from baseline to Month 4 and Month 8, the MFSO® hair oil treatment group was better than each of

the other two treatment groups by at least 117.6 percent
and 234.9 percent for the Healthy Hair Index 75 and 316.5 percent and 312 percent for the Healthy Hair Index 50 values, respectively, thereby achieving the primary efficacy objective. Subjects favored the MFSO® hair oil treatment, rating it as safe, effective, and aesthetically pleasing. Conclusions:
The MFSO hair oil product is a safe and effective option for the treatment of women suffering from hair breakage and damaged hair.